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KMID : 0545119960060030219
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Journal of Microbiology and Biotechnology
1996 Volume.6 No. 3 p.219 ~ p.220
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Linkage of the Kanamycin Resistance Gene with the Streptothricin Resistance Gene in Staphylococcus aureus SA2
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Shin, Chul Kyo
Im, Sung Hwan/Kim, Woo Koo/Moon, Kyung Ho
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Abstract
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The pKH2 isolated from the multidrug-resistant Staphylococcus aureus SA2 is a 40.98-kb plasmid and mediates resistance to ampicillin, clindamycin, erythromycin, kanamycin, and streptomycin. The 3.4-kb HindⅢ fragment conferring kanamycin resistance was cloned from the pKH2 into pBluescript Ⅱ KS^+ and partial sequence determination of that fragment was carried out. Sequence analysis revealed that the kanamycin resistance gene which encoded aminoglycoside 3´-phosphotransferase was linked to the streptothricin resistance gene. But a nonsense mutation was found in the streptothricin resistance gene and this mutation resulted in a truncated protein of streptothricin acetyltransferase. Homology comparison with nucleotide sequence databases revealed that the 3.4-kb HindⅢ fragment of pKH2 had been derived not from S. aureus but from Gram-negative Campylobacter coli.
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